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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD300A Recombinant antibody (84674-4-RR)

    WB analysis using 84674-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84674-4-RR (CD300A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CD300A Recombinant antibody (84674-4-RR)

    IF Staining of THP-1 using 84674-4-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD300A antibody (84674-4-RR, Clone: 242131H5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84674-4-RR

    Affinity and Kinetic Characterization of 84674-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84674-4-RR against Human CD300A were performed. The affinity constant is below 1 pM.

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CD300a Recombinant antibody

CD300a Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84674-4-RR
Clone No.242131H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

CMRF 35 H9, CLM-8, CLM 8, CD300a molecule, CD300 antigen-like family member A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTHP-1 cells, HL-60 cells
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84674-4-RR targets CD300a in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD300a molecule
Calculated molecular weight33 kDa
Observed molecular weight40-70 kDa
GenBank accession numberNM_007261.4
Gene Symbol CD300a
Gene ID (NCBI) 11314
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UGN4-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human CD300a is a transmembrane protein, with an immunoglobulin (Ig)V-like extracellular domain and a cytoplasmic tail containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs), providing the receptor with an inhibitory capacity. The relevance of the CD300a molecule in several pathological conditions has been highlighted by multiple studies. (PMID: 37762055)

Protocols

Product Specific Protocols
WB protocol for CD300a antibody 84674-4-RRDownload protocol
IF protocol for CD300a antibody 84674-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols