Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, U-937 cells |
| Positive IP detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
13334-1-AP targets CD300LF in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CD300LF fusion protein Ag4150 相同性解析による交差性が予測される生物種 |
| Full Name | CD300 molecule-like family member f |
| Calculated molecular weight | 290 aa, 32 kDa |
| Observed molecular weight | 30-34 kDa |
| GenBank accession number | BC028199 |
| Gene Symbol | CD300LF |
| Gene ID (NCBI) | 146722 |
| RRID | AB_2877941 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TDQ1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD300LF, also known as CLM1 (CMRF35-like molecule 1), is a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. CD300LF is an inhibitory receptor that has been shown to inhibit various inflammatory processes in myeloid cells, such as macrophages and mast cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD300LF antibody 13334-1-AP | Download protocol |
| IP protocol for CD300LF antibody 13334-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
BMC Pulm Med Identification of new biomarkers for Acute Respiratory Distress Syndrome by expression-based genome-wide association study. | ||
J Inflamm Res Quercetin Attenuates MRGPRX2-Mediated Mast Cell Degranulation via the MyD88/IKK/NF-κB and PI3K/AKT/ Rac1/Cdc42 Pathway |