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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD302 Monoclonal antibody

CD302 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B1E8

Cat no : 66640-1-Ig

Synonyms

BIMLEC, C type lectin BIMLEC, CD302, CD302 antigen, CD302 molecule, CLEC13A, DCL 1, DCL1, KIAA0022

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using CD302 Monoclonal antibody (66640-1-Ig)

    WB analysis of THP-1 using 66640-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66640-1-Ig (CD302 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using CD302 Monoclonal antibody (66640-1-Ig)

    WB analysis of RAW 264.7 using 66640-1-Ig

    LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66640-1-Ig (CD302 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of DC2.4 cells using CD302 Monoclonal antibody (66640-1-Ig)

    WB analysis of DC2.4 using 66640-1-Ig

    DC2.4 cells were subjected to SDS PAGE followed by western blot with 66640-1-Ig (CD302 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using CD302 Monoclonal antibody (66640-1-Ig)

    WB analysis of HL-60 using 66640-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 66640-1-Ig (CD302 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inTHP-1 cells, DC2.4 cells, HL-60 cells, LPS treated RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66640-1-Ig targets CD302 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD302 fusion protein Ag9873 相同性解析による交差性が予測される生物種
Full Name CD302 molecule
Calculated molecular weight170aa,19 kDa; 232aa,26 kDa
Observed molecular weight 30-32 kDa
GenBank accession numberBC020646
Gene symbol CD302
Gene ID (NCBI) 9936
RRIDAB_2881999
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CD302 antibody 66640-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols