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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using Anti-Human CD31 (WM-59) (65268-1-Ig)

    IF Staining of HUVEC using 65268-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CD31 antibody (65268-1-Ig, Clone: WM-59 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD31 (WM-59) (65268-1-Ig)

    FC experiment of human PBMCs using 65268-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD31 (65268-1-Ig, Clone:WM-59) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.

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Anti-Human CD31 (WM-59)

CD31 Monoclonal Antibody for IF/ICC, FC
Cat No. 65268-1-Ig
Clone No.WM-59

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

IF/ICC, FC

Synonyms

PECAM1, PECAM 1, PECA1, GPIIA', GPIIA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHUVEC cells
Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65268-1-Ig targets CD31 in IF/ICC, FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name platelet/endothelial cell adhesion molecule
Calculated molecular weight 83 kDa
GenBank accession numberBC022512
Gene Symbol CD31
Gene ID (NCBI) 5175
ENSEMBL Gene IDENSG00000261371
RRIDAB_2935139
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD31, also known as PECAM1 (Platelet endothelial cell adhesion molecule), is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. It is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, CD31 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Protocols

Product Specific Protocols
IF protocol for CD31 antibody 65268-1-IgDownload protocol
FC protocol for CD31 antibody 65268-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols