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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD31 (WM-59) Mouse IgG2a Recombinant An (65614-1-MR)

    FC experiment of human PBMCs using 65614-1-MR (same clone as 65614-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD31 (WM-59) Mouse IgG2a Recombinant Antibody (65614-1-MR, Clone: WM-59) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65614-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD31 (WM-59) Mouse IgG2a Recombinant An (65614-1-MR)

    FC experiment of human PBMCs using 65614-1-MR (same clone as 65614-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD31 (WM-59) Mouse IgG2a Recombinant Antibody (65614-1-MR, Clone: WM-59) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65614-1-PBS in a different storage buffer formulation.

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Anti-Human CD31 (WM-59) Mouse IgG2a Recombinant Antibody, PBS Only

CD31 Uni-rAbTM Recombinant Antibody for FC
Cat No. 65614-1-PBS
Clone No.WM-59

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

PECAM1, PECAM 1, PECA1, GPIIA', GPIIA

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65614-1-PBS targets CD31 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name platelet/endothelial cell adhesion molecule
Calculated molecular weight 83 kDa
GenBank accession numberBC022512
Gene Symbol CD31
Gene ID (NCBI) 5175
ENSEMBL Gene IDENSG00000261371
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD31, also known as PECAM1 (Platelet endothelial cell adhesion molecule), is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. It is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, CD31 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).