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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD31 Recombinant antibody, PBS Only

CD31 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4N13

Cat no : 80530-1-PBS

Synonyms

PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD31 Recombinant antibody (80530-1-RR)

    WB analysis using 80530-1-RR (same clone as 80530-1-PBS)

    Untreated and PNGase F-treated lysates of human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 80530-1-RR (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80530-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD31 Recombinant antibody (80530-1-RR)

    WB analysis using 80530-1-RR (same clone as 80530-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80530-1-RR (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80530-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of bEnd.3 cells using CD31 Recombinant antibody (80530-1-RR)

    IF Staining of bEnd.3 using 80530-1-RR (same clone as 80530-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD31 antibody (80530-1-RR, Clone: 4N13 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 80530-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80530-1-RR

    Affinity and Kinetic Characterization of 80530-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80530-1-RR against Human CD31 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80530-1-PBS targets CD31 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CD31 fusion protein Ag1787 相同性解析による交差性が予測される生物種
Full Name platelet/endothelial cell adhesion molecule
Calculated molecular weight 83 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC022512
Gene symbol CD31
Gene ID (NCBI) 5175
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).