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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD320 Polyclonal antibody

CD320 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24145-1-AP

Synonyms

8D6, 8D6 antigen, 8D6A, CD320, CD320 antigen, CD320 molecule, FDC signaling molecule 8D6, FDC SM 8D6, TCblR, Transcobalamin receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD320 Polyclonal antibody (24145-1-AP)

    WB analysis using 24145-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24145-1-AP (CD320 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHL-60 cells, HeLa cells, K-562 cells, Raji cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24145-1-AP targets CD320 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD320 fusion protein Ag21445 相同性解析による交差性が予測される生物種
Full Name CD320 molecule
Calculated molecular weight 29 kDa
Observed molecular weight70 kDa
GenBank accession numberBC000668
Gene symbol CD320
Gene ID (NCBI) 51293
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD320, also known as 8D6, 8D6A or TCblR, is a single-pass type I membrane protein containing 2 LDL-receptor class A domains. Expressed abundantly on follicular dendritic cells (FDCs), CD320 has been shown to enhance proliferation of germinal center (GC) B cells. It is the receptor for cellular uptake of transcobalamin-bound cobalamin. Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC). CD320 has a calculated molecular mass of 29 kDa.The apparent molecular weight detected by this antibody is 70 kDa. The aberrant mobility of the protein by SDS-PAGE is probably caused by extensive complex glycosylation (PMID: 18779389; 23415653).

Protocols

Product Specific Protocols
WB protocol for CD320 antibody 24145-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols