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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD320 Polyclonal antibody (24145-1-AP)

    WB analysis using 24145-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24145-1-AP (CD320 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD320 Polyclonal antibody (24145-1-AP)

    IHC staining of human tonsillitis using 24145-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24145-1-AP (CD320 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CD320 Polyclonal antibody (24145-1-AP)

    IF Staining of Raji using 24145-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD320 antibody (24145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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CD320 Polyclonal antibody

CD320 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24145-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Transcobalamin receptor, TCblR, FDC-SM-8D6, FDC-signaling molecule 8D6, 8D6A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells, HeLa cells, K-562 cells, Raji cells, human placenta tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24145-1-AP targets CD320 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD320 fusion protein Ag21445 相同性解析による交差性が予測される生物種
Full Name CD320 molecule
Calculated molecular weight 29 kDa
Observed molecular weight70 kDa
GenBank accession numberBC000668
Gene Symbol CD320
Gene ID (NCBI) 51293
RRIDAB_3669431
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9NPF0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD320, also known as 8D6, 8D6A or TCblR, is a single-pass type I membrane protein containing 2 LDL-receptor class A domains. Expressed abundantly on follicular dendritic cells (FDCs), CD320 has been shown to enhance proliferation of germinal center (GC) B cells. It is the receptor for cellular uptake of transcobalamin-bound cobalamin. Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC). CD320 has a calculated molecular mass of 29 kDa.The apparent molecular weight detected by this antibody is 70 kDa. The aberrant mobility of the protein by SDS-PAGE is probably caused by extensive complex glycosylation (PMID: 18779389; 23415653).

Protocols

Product Specific Protocols
WB protocol for CD320 antibody 24145-1-APDownload protocol
IHC protocol for CD320 antibody 24145-1-APDownload protocol
IF protocol for CD320 antibody 24145-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols