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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD32A/C Polyclonal antibody (25323-1-AP)

    WB analysis using 25323-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25323-1-AP (CD32A/C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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CD32A/C Polyclonal antibody

CD32A/C Polyclonal Antibody for WB, ELISA
Cat No. 25323-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CD32, CD32C, FcgammaRIIC, FCGR2B, FCGR2C, FcRIIC, hFcRII C

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inK-562 cells, U-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25323-1-AP targets CD32A/C in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD32A/C fusion protein Ag18123 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgG, low affinity IIc, receptor for (CD32)
Calculated molecular weight 323 aa, 36 kDa
Observed molecular weight40 kDa
GenBank accession numberBC137397
Gene Symbol CD32A/C
Gene ID (NCBI) 9103
RRIDAB_3085785
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31995
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IgG Fc receptors (FcγRs) play important roles in immune responses. The low-affinity IgG Fc receptor, FcγRII (CD32), consists of a family of primarily cell membrane receptor proteins: CD32A, CD32B, and CD32C. They are encoded by the mRNA splice variants of three highly related genes: FCGR2A, FCGR2B, and FCGR2C (PMID: 30941127). CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. This antibody raised against 254-323 aa of human CD32C (FCGR2C) can recognize both CD32A and CD32C forms.

Protocols

Product Specific Protocols
WB protocol for CD32A/C antibody 25323-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols