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Validation Data Gallery

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  • Western Blot (WB) analysis of Mo7e cells using CD33 Monoclonal antibody (67135-2-Ig)

    WB analysis of Mo7e using 67135-2-Ig

    Mo7e cells were subjected to SDS PAGE followed by western blot with 67135-2-Ig (CD33 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using CD33 Monoclonal antibody (67135-2-Ig)

    WB analysis of U-937 using 67135-2-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 67135-2-Ig (CD33 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using CD33 Monoclonal antibody (67135-2-Ig)

    WB analysis of THP-1 using 67135-2-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67135-2-Ig (CD33 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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CD33 Monoclonal antibody

CD33 Monoclonal Antibody for WB, ELISA
Cat No. 67135-2-Ig
Clone No.1B3G4

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Synonyms

CD33, CD33 molecule, FLJ00391, gp67, p67, SIGLEC 3, SIGLEC3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 1000 ug/ml
20 μL, 1000 ug/ml
150 μL, 1000 ug/ml

¥67,000/ 150 μL

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMo7e cells, THP-1 cells, U-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67135-2-Ig targets CD33 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD33 fusion protein Ag11402 相同性解析による交差性が予測される生物種
Full Name CD33 molecule
Calculated molecular weight 364 aa, 40 kDa
Observed molecular weight 67-70 kDa
GenBank accession numberBC028152
Gene Symbol CD33
Gene ID (NCBI) 945
RRIDAB_3085036
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20138
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.

Protocols

Product Specific Protocols
WB protocol for CD33 antibody 67135-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols