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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD33 Recombinant antibody

CD33 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241711D2

Cat no : 84341-3-RR

Synonyms

SIGLEC 3, Sialic acid-binding Ig-like lectin 3, p67, gp67, CD33 molecule

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD33 Recombinant antibody (84341-3-RR)

    WB analysis using 84341-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84341-3-RR (CD33 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD33 Recombinant antibody (84341-3-RR)

    IHC staining of human tonsillitis using 84341-3-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84341-3-RR (CD33 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD33 Recombinant antibody (84341-3-RR)

    IHC staining of human tonsillitis using 84341-3-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84341-3-RR (CD33 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inTHP-1 cells, K-562 cells, U-937 cells, HL-60 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84341-3-RR targets CD33 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name CD33 molecule
Calculated molecular weight 364 aa, 40 kDa
Observed molecular weight55-75 kDa
GenBank accession numberBC028152
Gene symbol CD33
Gene ID (NCBI) 945
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.

Protocols

Product Specific Protocols
WB protocol for CD33 antibody 84341-3-RRDownload protocol
IHC protocol for CD33 antibody 84341-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols