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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD34 Polyclonal antibody

CD34 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 31120-1-AP

Synonyms

AU040960, Cd34, CD34 antigen

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD34 Polyclonal antibody (31120-1-AP)

    IHC staining of mouse spleen using 31120-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31120-1-AP (CD34 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD34 Polyclonal antibody (31120-1-AP)

    IHC staining of mouse spleen using 31120-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31120-1-AP (CD34 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CD34 Polyclonal antibody (31120-1-AP)

    IHC staining of mouse testis using 31120-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31120-1-AP (CD34 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CD34 Polyclonal antibody (31120-1-AP)

    IHC staining of mouse testis using 31120-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31120-1-AP (CD34 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CD34 Polyclonal antibody (31120-1-AP)

    IHC staining of mouse kidney using 31120-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31120-1-AP (CD34 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CD34 Polyclonal antibody (31120-1-AP)

    IHC staining of mouse kidney using 31120-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31120-1-AP (CD34 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inmouse spleen tissue, mouse kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31120-1-AP targets CD34 in IHC, ELISA applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD34 antigen
Calculated molecular weight41 kDa
GenBank accession numberNM_133654
Gene symbol Cd34
Gene ID (NCBI) 12490
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

Protocols

Product Specific Protocols
IHC protocol for CD34 antibody 31120-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols