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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD34 Polyclonal antibody

CD34 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 32211-1-AP

Synonyms

CD34 molecule

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD34 Polyclonal antibody (32211-1-AP)

    WB analysis using 32211-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 32211-1-AP (CD34 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD34 Polyclonal antibody (32211-1-AP)

    IHC staining of human placenta using 32211-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 32211-1-AP (CD34 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD34 Polyclonal antibody (32211-1-AP)

    IHC staining of human placenta using 32211-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 32211-1-AP (CD34 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHL-60 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32211-1-AP targets CD34 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD34 molecule
Calculated molecular weight41kDa
GenBank accession numberNM_001025109.2
Gene symbol CD34
Gene ID (NCBI) 947
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. The calculated molecular mass of human CD34 is 41 kDa, various forms with different molecular weights may be produced due to different glycosylation patterns and alternative splicing (PMID: 24375067; 15750786).

Protocols

Product Specific Protocols
WB protocol for CD34 antibody 32211-1-APDownload protocol
IHC protocol for CD34 antibody 32211-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols