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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD34 Recombinant antibody (84242-5-RR)

    WB analysis using 84242-5-RR (same clone as 84242-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84242-5-RR (CD34 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84242-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD34 Recombinant antibody (84242-5-RR)

    IHC staining of human placenta using 84242-5-RR (same clone as 84242-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84242-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD34 Recombinant antibody (84242-5-RR)

    IHC staining of human placenta using 84242-5-RR (same clone as 84242-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84242-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD34 Recombinant antibody (84242-5-RR)

    IHC staining of human colon cancer using 84242-5-RR (same clone as 84242-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84242-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD34 Recombinant antibody (84242-5-RR)

    IHC staining of human liver cancer using 84242-5-RR (same clone as 84242-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84242-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD34 Recombinant antibody (84242-5-RR)

    IHC staining of human liver cancer using 84242-5-RR (same clone as 84242-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84242-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD34 Recombinant antibody (84242-5-RR)

    IHC staining of human tonsillitis using 84242-5-RR (same clone as 84242-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84242-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD34 Recombinant antibody (84242-5-RR)

    IHC staining of human tonsillitis using 84242-5-RR (same clone as 84242-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84242-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD34 Recombinant antibody (84242-5-RR)

    IHC staining of human colon cancer using 84242-5-RR (same clone as 84242-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84242-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84242-5-RR

    Affinity and Kinetic Characterization of 84242-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84242-5-RR against Human CD34 were performed. The affinity constant is 16.3 nM.

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CD34 Recombinant antibody, PBS Only

CD34 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84242-5-PBS
Clone No.242069F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

242069F8, CD34 molecule

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84242-5-PBS targets CD34 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD34 molecule
Calculated molecular weight 41 kDa
Observed molecular weight105 kDa
GenBank accession numberBC039146
Gene Symbol CD34
Gene ID (NCBI) 947
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP28906
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.