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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD35 (E11) Mouse IgG2a Recombinant Antibody, PBS Only

CD35 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

E11

Cat no : 65622-1-PBS

Synonyms

KN, E11, CR1, Complement receptor type 1, C3BR

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD35 (E11) Mouse IgG2a Recombinant Anti (65622-1-MR)

    FC experiment of human PBMCs using 65622-1-MR (same clone as 65622-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD35 (E11) Mouse IgG2a Recombinant Antibody (65622-1-MR, Clone: E11) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65622-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD35 (E11) Mouse IgG2a Recombinant Anti (65622-1-MR)

    FC experiment of human PBMCs using 65622-1-MR (same clone as 65622-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD35 (E11) Mouse IgG2a Recombinant Antibody (65622-1-MR, Clone: E11) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65622-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65622-1-PBS targets CD35 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human CD35 from Acute monocytic leukemia cells and normal blood monocytes 相同性解析による交差性が予測される生物種
Full Name complement component (3b/4b) receptor 1 (Knops blood group)
Calculated molecular weight 224 kDa
GenBank accession numberNM_000573
Gene symbol CR1
Gene ID (NCBI) 1378
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes.