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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD35 Monoclonal antibody, PBS Only

CD35 Monoclonal Antibody for IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B8E6

Cat no : 68033-1-PBS

Synonyms

KN, CR1, Complement receptor type 1, C3BR, C3b/C4b receptor

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD35 Monoclonal antibody (68033-1-Ig)

    IHC staining of human tonsillitis using 68033-1-Ig (same clone as 68033-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68033-1-Ig (CD35 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68033-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD35 Monoclonal antibody (68033-1-Ig)

    IHC staining of human tonsillitis using 68033-1-Ig (same clone as 68033-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68033-1-Ig (CD35 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68033-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using CD35 Monoclonal antibody (68033-1-Ig)

    IF Staining of human appendicitis using 68033-1-Ig (same clone as 68033-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CD35 antibody (68033-1-Ig, Clone: 1B8E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68033-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using CD35 Monoclonal antibody (68033-1-Ig)

    IF Staining of human appendicitis using 68033-1-Ig (same clone as 68033-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CR1 antibody (68033-1-Ig, Clone: 1B8E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68033-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68033-1-PBS targets CD35 in IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD35 fusion protein Ag30268 相同性解析による交差性が予測される生物種
Full Name complement component (3b/4b) receptor 1 (Knops blood group)
Calculated molecular weight 224 kDa
GenBank accession numberNM_000573
Gene symbol CR1
Gene ID (NCBI) 1378
RRIDAB_2918775
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes. A soluble form of CD35 has been found in plasma (PMID: 3156931). It has been reported that soluble CD35 is produced by a proteolytic cleavage in the C-terminal region of CD35 transmembrane domain (PMID: 9405292).