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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD36 Monoclonal antibody, PBS Only

CD36 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A8C5

Cat no : 66395-1-PBS

Synonyms

GPIV, GPIIIB, GP4, GP3B, Glycoprotein IIIb

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using CD36 Monoclonal antibody (66395-1-Ig)

    WB analysis of human heart using 66395-1-Ig (same clone as 66395-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66395-1-Ig (CD36 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human milk using CD36 Monoclonal antibody (66395-1-Ig)

    WB analysis of human milk using 66395-1-Ig (same clone as 66395-1-PBS)

    human milk were subjected to SDS PAGE followed by western blot with 66395-1-Ig (CD36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human spleen tissue using CD36 Monoclonal antibody (66395-1-Ig)

    WB analysis of human spleen using 66395-1-Ig (same clone as 66395-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66395-1-Ig (CD36 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using CD36 Monoclonal antibody (66395-1-Ig)

    WB analysis of human placenta using 66395-1-Ig (same clone as 66395-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66395-1-Ig (CD36 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CD36 Monoclonal antibody (66395-1-Ig)

    IHC staining of human spleen using 66395-1-Ig (same clone as 66395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66395-1-Ig (CD36 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CD36 Monoclonal antibody (66395-1-Ig)

    IHC staining of human spleen using 66395-1-Ig (same clone as 66395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66395-1-Ig (CD36 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CD36 Monoclonal antibody (66395-1-Ig)

    IHC staining of human heart using 66395-1-Ig (same clone as 66395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66395-1-Ig (CD36 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CD36 Monoclonal antibody (66395-1-Ig)

    IHC staining of human heart using 66395-1-Ig (same clone as 66395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66395-1-Ig (CD36 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CD36 Monoclonal antibody (66395-1-Ig)

    IF Staining of human liver cancer using 66395-1-Ig (same clone as 66395-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD36 antibody (66395-1-Ig, Clone: 1A8C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CD36 Monoclonal antibody (66395-1-Ig)

    IF Staining of THP-1 using 66395-1-Ig (same clone as 66395-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD36 antibody (66395-1-Ig, Clone: 1A8C5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66395-1-PBS targets CD36 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD36 fusion protein Ag13541 相同性解析による交差性が予測される生物種
Full Name CD36 molecule (thrombospondin receptor)
Calculated molecular weight 472 aa, 53 kDa
Observed molecular weight 88 kDa
GenBank accession numberBC008406
Gene symbol CD36
Gene ID (NCBI) 948
RRIDAB_2811030
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Function

CD36, also known as platelet glycoprotein 4, is an integral membrane glycoprotein that acts as a scavenger receptor. CD36 can bind to multiple ligands, including thrombospondin-1, collagen, oxidized phospholipids, oxidized low-density lipoprotein, and long-chain fatty acids. CD36 can also bind to erythrocytes parasitized by Plasmodium falciparum and apoptotic cells. CD36 mediates different biological processes, acting as a signaling hub in angiogenesis, inflammatory response, and fatty acid metabolism.

Tissue specificity

CD36 is present on the surface of various cells types, such as adipocytes, monocytes, macrophages, platelets, microvascular endothelial cells, dendritic cells, and hematopoietic precursors of red cells.

Involvement in disease

  • Mutations in CD36 can give rise to platelet glycoprotein IV deficiency, a type of macrothrombocytopenia.

  • Polymorphisms in CD36 can increase susceptibility to malaria.

  • AAGIC haplotype at the CD36 locus increases the risk of coronary heart disease.

  • Disruption of CD36-dependent pathways and certain SNPs in the CD36 gene are attributed to impaired fatty acid metabolism, glucose intolerance, Alzheimer's disease, atherosclerosis, arterial hypertension, diabetes, and cardiomyopathy.

Isoforms

Apart from the full-length protein (isoform 1), one additional shorter isoform has been reported (PMID: 7509795). Other alternative isoforms have also been detected on the mRNA level (PMID: 17673938).

Post-translational modifications

The extracellular domain of CD36 is extensively glycosylated. Glycosylation is needed for the transport of CD36 to the plasma membrane, as well as mediating recognition and binding to ligands. Cytoplasmic tails of transmembrane domains can be phosphorylated and play a role in signal transduction. Intracellular domains can be additionally acetylated, ubiquitinated, and palmitoylated (PMID: 28919632).

Cellular localization

CD36 is present on the cell surface.