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  • Featured Product
  • KD/KO Validated

CD38 Monoclonal antibody, PBS Only

CD38 Monoclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3C6G4

Cat no : 60006-1-PBS

Synonyms

ADP ribosyl cyclase 1, cADPr hydrolase 1, CD38, CD38 molecule, Cyclic ADP ribose hydrolase 1, T10

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD38 Monoclonal antibody (60006-1-Ig)

    WB analysis of Jurkat using 60006-1-Ig (same clone as 60006-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60006-1-Ig (CD38 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60006-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using CD38 Monoclonal antibody (60006-1-Ig)

    WB analysis of Raji using 60006-1-Ig (same clone as 60006-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 60006-1-Ig (CD38 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60006-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CD38 Monoclonal antibody (60006-1-Ig)

    IF Staining of NIH/3T3 using 60006-1-Ig (same clone as 60006-1-PBS)

    Immunofluorescent analysis of NIH/3T3 cells using 60006-1-Ig (CD38 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 60006-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Daudi cells using CD38 Monoclonal antibody (60006-1-Ig)

    FC experiment of Daudi using 60006-1-Ig (same clone as 60006-1-PBS)

    1X10^6 Daudi cells were intracellularly stained with 0.4 ug Anti-Human CD38 (60006-1-Ig, Clone:3C6G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60006-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60006-1-PBS targets CD38 in WB, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD38 fusion protein Ag0487 相同性解析による交差性が予測される生物種
Full Name CD38 molecule
Calculated molecular weight 300 aa, 34 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC007964
Gene symbol CD38
Gene ID (NCBI) 952
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).