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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD38 Monoclonal antibody (60006-1-Ig)

    WB analysis using 60006-1-Ig (same clone as 60006-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60006-1-Ig (CD38 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60006-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD38 Monoclonal antibody (60006-1-Ig)

    WB analysis using 60006-1-Ig (same clone as 60006-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60006-1-Ig (CD38 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60006-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD38 Monoclonal antibody (60006-1-Ig)

    IF Staining of mouse spleen using 60006-1-Ig (same clone as 60006-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD38 antibody (60006-1-Ig, Clone: 3C6G4 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60006-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD38 Monoclonal antibody (60006-1-Ig)

    IF Staining of mouse spleen using 60006-1-Ig (same clone as 60006-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD38 antibody (60006-1-Ig, Clone: 3C6G4 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60006-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CD38 Monoclonal antibody (60006-1-Ig)

    IF Staining of Raji using 60006-1-Ig (same clone as 60006-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD38 antibody (60006-1-Ig, Clone: 3C6G4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60006-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CD38 Monoclonal antibody, PBS Only

CD38 Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA
Cat No. 60006-1-PBS
Clone No.3C6G4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF/ICC, IF-P, ELISA

Synonyms

ADPRC 1, ADP ribosyl cyclase 1, 3C6G4, 2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60006-1-PBS targets CD38 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD38 fusion protein Ag0487 相同性解析による交差性が予測される生物種
Full Name CD38 molecule
Calculated molecular weight 300 aa, 34 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC007964
Gene Symbol CD38
Gene ID (NCBI) 952
ENSEMBL Gene IDENSG00000004468
RRIDAB_716994
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP28907
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).