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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD39/ENTPD1 Monoclonal antibody (60323-1-Ig)

    WB analysis using 60323-1-Ig (same clone as 60323-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60323-1-Ig (CD39/ENTPD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60323-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD39/ENTPD1 Monoclonal antibody (60323-1-Ig)

    IHC staining of human placenta using 60323-1-Ig (same clone as 60323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60323-1-Ig (CD39/ENTPD1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60323-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD39/ENTPD1 Monoclonal antibody (60323-1-Ig)

    IHC staining of human placenta using 60323-1-Ig (same clone as 60323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60323-1-Ig (CD39/ENTPD1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60323-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD39/ENTPD1 Monoclonal antibody (60323-1-Ig)

    IF Staining of HeLa using 60323-1-Ig (same clone as 60323-1-PBS)

    Immunofluorescent analysis of HeLa cells using 60323-1-Ig (ENTPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60323-1-PBS in a different storage buffer formulation.

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CD39/ENTPD1 Monoclonal antibody, PBS Only

CD39/ENTPD1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60323-1-PBS
Clone No.2D2H6

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ATPDase, CD39, CD39/ENTPD1, Ecto apyrase, Ecto ATP diphosphohydrolase 1, Ecto ATPase 1, Ecto ATPDase 1, ENTPD1, e-NTPDase, NTPDase 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60323-1-PBS targets CD39/ENTPD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD39/ENTPD1 fusion protein Ag6165 相同性解析による交差性が予測される生物種
Full Name ectonucleoside triphosphate diphosphohydrolase 1
Calculated molecular weight 58 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC047664
Gene Symbol CD39
Gene ID (NCBI) 953
RRIDAB_2881434
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49961
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named as CD39, NTPDase 1, Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission.It is a noncovalent tetrameric protein and that detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ).And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). This protein has 6 glycosylation sites and 3 isoforms(58 kDa, 59 kDa and 34 kDa) produced by alternative splicing. The soluble isoform of CD39(38-478 amino acid) locates to the extracellular part and 60323-1-Ig can recognize the soluble form of CD39 (38-478aa) molecule.