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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD39/ENTPD1 Rabbit Recombinant Antibody, PBS Only

CD39/ENTPD1 Recombinant Antibody for FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241292G7

Cat no : 98102-1-PBS

Synonyms

ENTPD1, EC:3.6.1.5, CD39, ATP-DPH, ATPDase

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP01143-1

    Sandwich ELISA standard curve of MP01143-1

    Sandwich ELISA standard curve of MP01143-3, Human CD39/ENTPD1 Recombinant Matched Antibody Pair - PBS only. 98102-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02313. 84235-2-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL.

  • Sample test of MP01143-1

    Sample test of MP01143-1

    Plasma of six individual healthy human donors was measured. The CD39/ENTPD1 concentration of detected samples was determined to be 179.9 pg/mL with a range of 134.2-243.6 ng/mL.

  • Sample test of MP01143-1

    Sample test of MP01143-1

    Serum of six individual healthy human donors was measured. The CD39/ENTPD1 concentration of detected samples was determined to be 373.5 pg/mL with a range of 283.0-450.4 pg/mL.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD39/ENTPD1 Rabbit Recombinant Antibody (98102-1-RR)

    FC experiment of human PBMCs using 98102-1-RR (same clone as 98102-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD39/ENTPD1 Rabbit Recombinant Antibody (98102-1-RR, Clone: 241292G7) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD4 (OKT4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98102-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD39/ENTPD1 Rabbit Recombinant Antibody (98102-1-RR)

    FC experiment of human PBMCs using 98102-1-RR (same clone as 98102-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD39/ENTPD1 Rabbit Recombinant Antibody (98102-1-RR, Clone: 241292G7) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98102-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01143-1

    Cytometric bead array standard curve of MP01143-1

    Cytometric bead array standard curve of MP01143-1, ENTPD1/CD39 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98102-1-PBS. Detection antibody: 84235-2-PBS. Standard: RP02313. Range: 0.156-20 ng/mL.

  • Cytometric bead array standard curve of MP01143-2

    Cytometric bead array standard curve of MP01143-2

    Cytometric bead array standard curve of MP01143-2, ENTPD1/CD39 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98102-1-PBS. Detection antibody: 84235-1-PBS. Standard: RP02313. Range: 0.156-20 ng/mL.

  • Cytometric bead array standard curve of MP01143-3

    Cytometric bead array standard curve of MP01143-3

    Cytometric bead array standard curve of MP01143-3, ENTPD1/CD39 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84235-3-PBS. Detection antibody: 98102-1-PBS. Standard: RP02313. Range: 0.156-20 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98102-1-PBS targets CD39/ENTPD1 as part of a matched antibody pair:

MP01143-1: 98102-1-PBS capture and 84235-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01143-2: 98102-1-PBS capture and 84235-1-PBS detection (validated in Cytometric bead array)

MP01143-3: 84235-3-PBS capture and 98102-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name ectonucleoside triphosphate diphosphohydrolase 1
Calculated molecular weight 58 kDa
GenBank accession numberBC047664
Gene symbol CD39
Gene ID (NCBI) 953
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ENTPD1 (Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers (PMID: 9733785 ).