Validation Data Gallery

Filter: AllWBIPIHCIF-P

WB analysis using 17617-1-AP
Jurkat cells (CD3+), MOLT-4 cells (CD3+), U-937 cells (CD3-), Raji cells (CD3-), mouse thymus tissue, and rat thymus tissue were subjected to SDS PAGE followed by western blot with 17617-1-AP (CD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 17617-1-AP
WB result of CD3 antibody (17617-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD3 transfected Jurkat cells.
WB analysis of pig spleen using 17617-1-AP
pig spleen tissue were subjected to SDS PAGE followed by western blot with 17617-1-AP (CD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 17617-1-AP
IP result of anti-CD3 (IP:17617-1-AP, 4ug; Detection:17617-1-AP 1:1000) with Jurkat cells lysate 3600ug.
IHC staining of human tonsillitis using 17617-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 17617-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 17617-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 17617-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 17617-1-AP
Immunohistochemical analysis of paraffin-embedded human angioimmunoblastic T-cell lymphoma using 17617-1-AP (CD3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lymphoma using 17617-1-AP
Immunohistochemical analysis of paraffin-embedded human angioimmunoblastic T-cell lymphoma using 17617-1-AP (CD3 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung cancer using 17617-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 17617-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 17617-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 17617-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 17617-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 17617-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 17617-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 17617-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat thymus using 17617-1-AP
Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat thymus using 17617-1-AP
Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 17617-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human tonsillitis using 17617-1-AP
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (17617-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CD20 antibody (60271-1-Ig, Clone: 4A7G3, red).
IF Staining of human tonsillitis using 17617-1-AP
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (17617-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CD20 antibody (60271-1-Ig, Clone: 4A7G3, red).
IF Staining of mouse spleen using 17617-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CD3 antibody (17617-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse spleen using 17617-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CD3 antibody (17617-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of human tonsillitis using 17617-1-AP
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (17617-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CD20 antibody (60271-1-Ig, Clone: 4A7G3, red).

Featured Product
KD/KO Validated

CD3 Polyclonal antibody

CD3 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 17617-1-AP

Publications(140)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, IP, ELISA, Cell treatment

Synonyms

CD3E, CD3 epsilon, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, T3E

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (140)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, pig spleen tissue, MOLT-4 cells, mouse thymus tissue, rat thymus tissue
Positive IP detected in	Jurkat cells
Positive IHC detected in	human tonsillitis tissue, mouse spleen tissue, human lymphoma tissue, human lung cancer tissue, human breast cancer tissue, mouse colon tissue, human pancreas cancer tissue, rat thymus tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human tonsillitis tissue, mouse spleen tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 7 publications below
IHC	See 81 publications below
IF	See 47 publications below
FC	See 4 publications below

Product Information

17617-1-AP targets CD3 in WB, IHC, IF-P, IP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CD3 fusion protein Ag11797 相同性解析による交差性が予測される生物種
Full Name	CD3e molecule, epsilon (CD3-TCR complex)
Calculated molecular weight	207 aa, 23 kDa
Observed molecular weight	23-25 kDa
GenBank accession number	BC049847
Gene Symbol	CD3
Gene ID (NCBI)	916
ENSEMBL Gene ID	ENSG00000198851
RRID	AB_1939430
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P07766
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a polyclonal antibody directed against the epsilon chain of human CD3 molecule.

Protocols

Product Specific Protocols
WB protocol for CD3 antibody 17617-1-AP	Download protocol
IHC protocol for CD3 antibody 17617-1-AP	Download protocol
IF protocol for CD3 antibody 17617-1-AP	Download protocol
IP protocol for CD3 antibody 17617-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
pig	IF	Nature Injectable ultrasonic sensor for wireless monitoring of intracranial signals Authors - Hanchuan Tang View Article
human	IHC	Blood C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse Authors - Li-Xue Yang View Article
human	IHC	Nat Commun Engineered triple inhibitory receptor resistance improves anti-tumor CAR-T cell performance via CD56. Authors - Fan Zou View Article
rat	IHC	J Adv Res Zearalenone-14-glucoside specifically promotes dysplasia of Gut-Associated Lymphoid Tissue: A natural product for constructing intestinal nodular lymphatic hyperplasia model Authors - Haonan Ruan View Article
human	IHC	Cancer Res M6A Demethylase ALKBH5 Regulates PD-L1 Expression and Tumor Immunoenvironment in Intrahepatic Cholangiocarcinoma. Authors - Xinyao Qiu View Article
		Acta Biomater Simultaneous targeting of TGF-β1/PD-L1 via a hydrogel-nanoparticle system to remodel the ECM and immune microenvironment for limiting adhesion formation Authors - Qian Qian Yang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 17617-1-AP

WB analysis of Jurkat using 17617-1-AP

WB analysis of pig spleen using 17617-1-AP

IP experiment of Jurkat using 17617-1-AP

IHC staining of human tonsillitis using 17617-1-AP

IHC staining of human tonsillitis using 17617-1-AP

IHC staining of mouse spleen using 17617-1-AP

IHC staining of mouse spleen using 17617-1-AP

IHC staining of human lymphoma using 17617-1-AP

IHC staining of human lymphoma using 17617-1-AP

IHC staining of human lung cancer using 17617-1-AP

IHC staining of human lung cancer using 17617-1-AP

IHC staining of human lung cancer using 17617-1-AP

IHC staining of human lung cancer using 17617-1-AP

IHC staining of human breast cancer using 17617-1-AP

IHC staining of human breast cancer using 17617-1-AP

IHC staining of human pancreas cancer using 17617-1-AP

IHC staining of human pancreas cancer using 17617-1-AP

IHC staining of rat thymus using 17617-1-AP

IHC staining of rat thymus using 17617-1-AP

IHC staining of mouse colon using 17617-1-AP

IF Staining of human tonsillitis using 17617-1-AP

IF Staining of human tonsillitis using 17617-1-AP

IF Staining of mouse spleen using 17617-1-AP

IF Staining of mouse spleen using 17617-1-AP

IF Staining of human tonsillitis using 17617-1-AP

CD3 Polyclonal antibody

CD3 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Injectable ultrasonic sensor for wireless monitoring of intracranial signals

C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse

Engineered triple inhibitory receptor resistance improves anti-tumor CAR-T cell performance via CD56.

Zearalenone-14-glucoside specifically promotes dysplasia of Gut-Associated Lymphoid Tissue: A natural product for constructing intestinal nodular lymphatic hyperplasia model

M6A Demethylase ALKBH5 Regulates PD-L1 Expression and Tumor Immunoenvironment in Intrahepatic Cholangiocarcinoma.

Simultaneous targeting of TGF-β1/PD-L1 via a hydrogel-nanoparticle system to remodel the ECM and immune microenvironment for limiting adhesion formation