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Species Reactivity

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  • Featured Product
  • KD/KO Validated

CD3 Polyclonal antibody

CD3 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, IP, ELISA, Cell treatment

Conjugate

Unconjugated

Cat no : 17617-1-AP

Synonyms

CD3E, CD3 epsilon, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, T3E

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD3 Polyclonal antibody (17617-1-AP)

    WB analysis of Jurkat using 17617-1-AP

    WB result of CD3 antibody (17617-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD3 transfected Jurkat cells.

  • Western Blot (WB) analysis of various lysates using CD3 Polyclonal antibody (17617-1-AP)

    WB analysis using 17617-1-AP

    Jurkat cells (CD3+), MOLT-4 cells (CD3+), U-937 cells (CD3-), Raji cells (CD3-), mouse thymus tissue, and rat thymus tissue were subjected to SDS PAGE followed by western blot with 17617-1-AP (CD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig spleen tissue using CD3 Polyclonal antibody (17617-1-AP)

    WB analysis of pig spleen using 17617-1-AP

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 17617-1-AP (CD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using CD3 Polyclonal antibody (17617-1-AP)

    IP experiment of Jurkat using 17617-1-AP

    IP result of anti-CD3 (IP:17617-1-AP, 4ug; Detection:17617-1-AP 1:1000) with Jurkat cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD3 Polyclonal antibody (17617-1-AP)

    IHC staining of human tonsillitis using 17617-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD3 Polyclonal antibody (17617-1-AP)

    IHC staining of human tonsillitis using 17617-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD3 Polyclonal antibody (17617-1-AP)

    IHC staining of mouse spleen using 17617-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD3 Polyclonal antibody (17617-1-AP)

    IHC staining of mouse spleen using 17617-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CD3 Polyclonal antibody (17617-1-AP)

    IHC staining of human pancreas cancer using 17617-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CD3 Polyclonal antibody (17617-1-AP)

    IHC staining of human pancreas cancer using 17617-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat thymus tissue using CD3 Polyclonal antibody (17617-1-AP)

    IHC staining of rat thymus using 17617-1-AP

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat thymus tissue using CD3 Polyclonal antibody (17617-1-AP)

    IHC staining of rat thymus using 17617-1-AP

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD3 Polyclonal antibody (17617-1-AP)

    IHC staining of human lung cancer using 17617-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD3 Polyclonal antibody (17617-1-AP)

    IHC staining of human lung cancer using 17617-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD3 Polyclonal antibody (17617-1-AP)

    IHC staining of human lung cancer using 17617-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD3 Polyclonal antibody (17617-1-AP)

    IHC staining of human lung cancer using 17617-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD3 Polyclonal antibody (17617-1-AP)

    IHC staining of human breast cancer using 17617-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD3 Polyclonal antibody (17617-1-AP)

    IHC staining of human breast cancer using 17617-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using CD3 Polyclonal antibody (17617-1-AP)

    IHC staining of mouse colon using 17617-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD3 Polyclonal antibody (17617-1-AP)

    IHC staining of human lymphoma using 17617-1-AP

    Immunohistochemical analysis of paraffin-embedded human angioimmunoblastic T-cell lymphoma using 17617-1-AP (CD3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD3 Polyclonal antibody (17617-1-AP)

    IHC staining of human lymphoma using 17617-1-AP

    Immunohistochemical analysis of paraffin-embedded human angioimmunoblastic T-cell lymphoma using 17617-1-AP (CD3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD3 Polyclonal antibody (17617-1-AP)

    IF Staining of human tonsillitis using 17617-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (17617-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CD20 antibody (60271-1-Ig, Clone: 4A7G3, red).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD3 Polyclonal antibody (17617-1-AP)

    IF Staining of human tonsillitis using 17617-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (17617-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CD20 antibody (60271-1-Ig, Clone: 4A7G3, red).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD3 Polyclonal antibody (17617-1-AP)

    IF Staining of mouse spleen using 17617-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CD3 antibody (17617-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD3 Polyclonal antibody (17617-1-AP)

    IF Staining of mouse spleen using 17617-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CD3 antibody (17617-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD3 Polyclonal antibody (17617-1-AP)

    IF Staining of human tonsillitis using 17617-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (17617-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CD20 antibody (60271-1-Ig, Clone: 4A7G3, red).

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Tested Applications

Positive WB detected inJurkat cells, pig spleen tissue, MOLT-4 cells, mouse thymus tissue, rat thymus tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue, mouse spleen tissue, human lymphoma tissue, human lung cancer tissue, human breast cancer tissue, mouse colon tissue, human pancreas cancer tissue, rat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue, mouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17617-1-AP targets CD3 in WB, IHC, IF-P, IP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD3 fusion protein Ag11797 相同性解析による交差性が予測される生物種
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Calculated molecular weight 207 aa, 23 kDa
Observed molecular weight 23-25 kDa
GenBank accession numberBC049847
Gene symbol CD3
Gene ID (NCBI) 916
RRIDAB_1939430
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a polyclonal antibody directed against the epsilon chain of human CD3 molecule.

Protocols

Product Specific Protocols
WB protocol for CD3 antibody 17617-1-APDownload protocol
IHC protocol for CD3 antibody 17617-1-APDownload protocol
IF protocol for CD3 antibody 17617-1-APDownload protocol
IP protocol for CD3 antibody 17617-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigIF

Nature

Injectable ultrasonic sensor for wireless monitoring of intracranial signals

Authors - Hanchuan Tang
View Article
humanIHC

Blood

C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse

Authors - Li-Xue Yang
View Article
humanIHC

Nat Commun

Engineered triple inhibitory receptor resistance improves anti-tumor CAR-T cell performance via CD56.

Authors - Fan Zou
View Article
ratIHC

J Adv Res

Zearalenone-14-glucoside specifically promotes dysplasia of Gut-Associated Lymphoid Tissue: A natural product for constructing intestinal nodular lymphatic hyperplasia model

Authors - Haonan Ruan
View Article
humanIHC

Cancer Res

M6A Demethylase ALKBH5 Regulates PD-L1 Expression and Tumor Immunoenvironment in Intrahepatic Cholangiocarcinoma.

Authors - Xinyao Qiu
View Article
mouseIHC

Br J Pharmacol

Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A

Authors - Lu Zhang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Federica (Verified Customer) (12-08-2023)

Leica Bond RXm Red Chromogenic Kit Antigen Retrival ER1 (ph6) for 30 min

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: Tonsil
CD3 Antibody Immunohistochemistry validation (1:400 dilution) in Tonsil (Cat no:17617-1-AP)
FH

Niklas (Verified Customer) (07-31-2023)

works well

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: human heart
FH

Emma (Verified Customer) (11-29-2021)

This works really well by IF on FFPE tissue. We used a 1:50 dilution and Tris-EDTA antigen retrieval.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Prostate
CD3 Antibody Immunofluorescence validation (1:50 dilution) in Prostate (Cat no:17617-1-AP)