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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD3EAP Polyclonal antibody (31014-1-AP)

    WB analysis using 31014-1-AP (same clone as 31014-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31014-1-AP (CD3EAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31014-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CD3EAP Polyclonal antibody (31014-1-AP)

    IF Staining of U2OS using 31014-1-AP (same clone as 31014-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CD3EAP antibody (31014-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 31014-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD3EAP Polyclonal antibody (31014-1-AP)

    IF Staining of HeLa using 31014-1-AP (same clone as 31014-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD3EAP antibody (31014-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 31014-1-PBS in a different storage buffer formulation.

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CD3EAP Polyclonal antibody, PBS Only

CD3EAP Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 31014-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CD3E-associated protein, CAST, ASE-1, ASE1, Antisense to ERCC-1 protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31014-1-PBS targets CD3EAP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD3EAP fusion protein Ag34108 相同性解析による交差性が予測される生物種
Full Name CD3e molecule, epsilon associated protein
Calculated molecular weight55 kDa
Observed molecular weight80-90 kDa
GenBank accession numberNM_012099.1
Gene Symbol CD3EAP
Gene ID (NCBI) 10849
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO15446
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Human RNA polymerase I (Pol I)-specific subunit, previously identified as ASE-1 and as CD3ɛ-associated signal transducer (CAST), CD3EAP is a 55 kDa nucleolar autoantigen that has an apparent molecular mass of 90 kDa (PMID: 11199923). CD3EAP/hPAF49 contains a single tyrosine residue at position 82 (Tyr82), which is phosphorylated upon stimulation of the T-cell receptor. Western blot analysis detected CD3EAP at an apparent molecular mass of ~80-90 kDa.