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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD3EAP Polyclonal antibody (31162-1-AP)

    WB analysis using 31162-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31162-1-AP (CD3EAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CD3EAP Polyclonal antibody (31162-1-AP)

    IF Staining of A431 using 31162-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CD3EAP antibody (31162-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CD3EAP Polyclonal antibody

CD3EAP Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31162-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

A34.5, Antisense to ERCC 1 protein, ASE 1, ASE1, CAST, CD3 epsilon associated protein, CD3E associated protein, CD3EAP, PAF49

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, A549 cells, HeLa cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31162-1-AP targets CD3EAP in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD3EAP fusion protein Ag35074 相同性解析による交差性が予測される生物種
Full Name CD3e molecule, epsilon associated protein
Calculated molecular weight 510 aa, 55 kDa
Observed molecular weight80-90 kDa
GenBank accession numberBC038992
Gene Symbol CD3EAP
Gene ID (NCBI) 10849
RRIDAB_3669876
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO15446
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human RNA polymerase I (Pol I)-specific subunit, previously identified as ASE-1 and as CD3ɛ-associated signal transducer (CAST), CD3EAP is a 55 kDa nucleolar autoantigen that has an apparent molecular mass of 90 kDa (PMID: 11199923). CD3EAP/hPAF49 contains a single tyrosine residue at position 82 (Tyr82), which is phosphorylated upon stimulation of the T-cell receptor. Western blot analysis detected CD3EAP at an apparent molecular mass of ~80-90 kDa.

Protocols

Product Specific Protocols
WB protocol for CD3EAP antibody 31162-1-APDownload protocol
IF protocol for CD3EAP antibody 31162-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols