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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD4 (SK3), PBS Only

CD4 Monoclonal Antibody for FC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

SK3

Cat no : 65147-1-PBS

Synonyms

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, SK3, CD4mut, CD4 molecule

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP50077-1

    Sandwich ELISA standard curve of MP50077-1

    Sandwich ELISA standard curve of MP50077-1, CD4 Monoclonal Matched Antibody Pair - PBS only. 65147-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Eg0333. 68688-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD4 (SK3) (65147-1-Ig)

    FC experiment of human PBMCs using 65147-1-Ig (same clone as 65147-1-PBS)

    1X10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3 and 0.2 ug Anti-Human CD4 (65147-1-Ig, Clone:SK3) labeled with FlexAble CoraLite® Plus 750 Antibody Labeling Kit for Mouse IgG1 (KFA024) (right), or Mouse IgG1 Isotype Control (left). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65147-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD4 (SK3) (65147-1-Ig)

    FC experiment of human PBMCs using 65147-1-Ig (same clone as 65147-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD4 (65147-1-Ig, Clone:SK3) labeled with FlexAble CoraLite® Plus 750 Antibody Labeling Kit for Mouse IgG1 (KFA024) (red), or Mouse IgG1 Isotype Control (blue). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65147-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65147-1-PBS targets CD4 as part of a matched antibody pair:

MP50077-1: 65147-1-PBS capture and 68688-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Affinity
KD=8.82 x 10-11M
KOff=2.00 x 10-5M
KOn=2.26 x 105M
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD4 molecule
Calculated molecular weight 55 kDa
GenBank accession numberBC025782
Gene symbol CD4
Gene ID (NCBI) 920
RRIDAB_2918432
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).