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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD4 (OKT4) Rabbit Recombinant Antibody, PBS only

CD4 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

OKT4

Cat no : 65505-1-PBS

Synonyms

CD4, CD4 molecule, CD4mut

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD4 (OKT4) (65505-1-PBS)

    FC experiment of human PBMCs using 65505-1-PBS

    1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD4 Rabbit Recombinant Antibody (65505-1-PBS, Clone:OKT4) or Rabbit IgG control PolyAb, and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD4 (OKT4) (65505-1-PBS)

    FC experiment of human PBMCs using 65505-1-PBS

    1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD4 Rabbit Recombinant Antibody (65505-1-PBS, Clone:OKT4) or Rabbit IgG control PolyAb, and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD4 (OKT4) (65505-1-PBS)

    FC experiment of human PBMCs using 65505-1-PBS

    1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD4 Rabbit Recombinant Antibody (65505-1-PBS, Clone:OKT4) and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (left) or 0.1 ug Anti-Human CD4 Mouse Monoclonal Antibody (Clone:OKT4) and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000 (center and right). Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65505-1-PBS targets CD4 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Human peripheral T cells 相同性解析による交差性が予測される生物種
Full Name CD4 molecule
Calculated molecular weight 55 kDa
GenBank accession numberBC025782
Gene symbol CD4
Gene ID (NCBI) 920
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
FC protocol for CD4 antibody 65505-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols