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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD4 (OKT4) Mouse IgG2a Recombinant Antibody

CD4 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

OKT4

Cat no : 65603-1-MR

Synonyms

CD4, CD4 molecule, CD4mut

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD4 (OKT4) Mouse IgG2a Recombinant Anti (65603-1-MR)

    FC experiment of human PBMCs using 65603-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Mouse IgG2a Recombinant Antibody (65603-1-MR, Clone: OKT4) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD4 (OKT4) Mouse IgG2a Recombinant Anti (65603-1-MR)

    FC experiment of human PBMCs using 65603-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Mouse IgG2a Recombinant Antibody (65603-1-MR, Clone: OKT4) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD4 (OKT4) Mouse IgG2a Recombinant Anti (65603-1-MR)

    FC experiment of human PBMCs using 65603-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Mouse IgG2a Recombinant Antibody (65603-1-MR, Clone: OKT4) (left) or 0.25 ug Anti-Human CD4 (65134-1-Ig, Clone: OKT4) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65603-1-MR targets CD4 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD4 molecule
Calculated molecular weight 55 kDa
GenBank accession numberBC025782
Gene symbol CD4
Gene ID (NCBI) 920
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
FC protocol for CD4 antibody 65603-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols