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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD4 Monoclonal antibody, PBS Only

CD4 Monoclonal Antibody for IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H4A2

Cat no : 67786-1-PBS

Synonyms

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4mut, CD4 molecule, 2H4A2

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD4 Monoclonal antibody (67786-1-Ig)

    IHC staining of human tonsillitis using 67786-1-Ig (same clone as 67786-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD4 Monoclonal antibody (67786-1-Ig)

    IHC staining of human tonsillitis using 67786-1-Ig (same clone as 67786-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thymus tissue using CD4 Monoclonal antibody (67786-1-Ig)

    IHC staining of human thymus using 67786-1-Ig (same clone as 67786-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using CD4 Monoclonal antibody (67786-1-Ig)

    IHC staining of human colon using 67786-1-Ig (same clone as 67786-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using CD4 Monoclonal antibody (67786-1-Ig)

    IHC staining of human colon using 67786-1-Ig (same clone as 67786-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CD4 Monoclonal antibody (67786-1-Ig)

    IHC staining of human liver using 67786-1-Ig (same clone as 67786-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CD4 Monoclonal antibody (67786-1-Ig)

    IHC staining of human liver using 67786-1-Ig (same clone as 67786-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thymus tissue using CD4 Monoclonal antibody (67786-1-Ig)

    IHC staining of human thymus using 67786-1-Ig (same clone as 67786-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD4 Monoclonal antibody (67786-1-Ig)

    IF Staining of human tonsillitis using 67786-1-Ig (same clone as 67786-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD4 antibody (67786-1-Ig, Clone: 2H4A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67786-1-PBS targets CD4 in IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD4 fusion protein Ag30870 相同性解析による交差性が予測される生物種
Full Name CD4 molecule
Calculated molecular weight 55 kDa
GenBank accession numberBC025782
Gene symbol CD4
Gene ID (NCBI) 920
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).