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Validation Data Gallery

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  • Western Blot (WB) analysis of U-937 cells using CD4 Recombinant antibody (83513-6-RR)

    WB analysis of U-937 using 83513-6-RR (same clone as 83513-6-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 83513-6-RR (CD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83513-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83513-6-RR

    Affinity and Kinetic Characterization of 83513-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83513-6-RR against Human CD4 were performed. The affinity constant is 0.391 nM.

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CD4 Recombinant antibody, PBS Only

CD4 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83513-6-PBS
Clone No.240427B10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4mut, CD4 molecule, 240427B10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83513-6-PBS targets CD4 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD4 molecule
Calculated molecular weight 55 kDa
GenBank accession numberBC025782
Gene Symbol CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01730
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.