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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD4 Recombinant antibody, PBS Only

CD4 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240427E12

Cat no : 83513-7-PBS

Synonyms

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4mut, CD4 molecule, 240427E12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD4 Recombinant antibody (83513-7-RR)

    WB analysis using 83513-7-RR (same clone as 83513-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83513-7-RR (CD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83513-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CD4 Recombinant antibody (83513-7-RR)

    IF Staining of THP-1 using 83513-7-RR (same clone as 83513-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD4 antibody (83513-7-RR, Clone: 240427E12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83513-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83513-7-RR

    Affinity and Kinetic Characterization of 83513-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83513-7-RR against Human CD4 were performed. The affinity constant is 0.426 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83513-7-PBS is CD4 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD4 molecule
Calculated molecular weight 55 kDa
GenBank accession numberBC025782
Gene symbol CD4
Gene ID (NCBI) 920
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.