CoraLite® Plus 750 Anti-Human CD4 (OKT4)
CD4 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
FC
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
OKT4
Cat no : CL750-65134
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL750-65134 targets CD4 in FC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Human peripheral T cells 相同性解析による交差性が予測される生物種 |
| Full Name | CD4 molecule |
| Calculated molecular weight | 55 kDa |
| GenBank accession number | BC025782 |
| Gene symbol | CD4 |
| Gene ID (NCBI) | 920 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 750 CD4 antibody CL750-65134 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sareena (Verified Customer) (08-04-2023) | Stained PBMCs well as multiplex sample
|
FH Sareena (Verified Customer) (08-03-2023) | Worked well with compensation beads. quick delivery
|
FH Sareena (Verified Customer) (06-30-2023) | Performed well on the fortessa as well as spectral instruments when stained with compensation beads
|
FH Sunil (Verified Customer) (06-23-2023) | Works great and quick arrival
|
