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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CD40 Polyclonal antibody (12971-1-AP)

    WB analysis of HeLa using 12971-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12971-1-AP (CD40 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CD40 Polyclonal antibody (12971-1-AP)

    WB analysis of Jurkat using 12971-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12971-1-AP (CD40 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

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CD40 Polyclonal antibody

CD40 Polyclonal Antibody for ELISA
Cat No. 12971-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12971-1-AP targets CD40 in ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD40 fusion protein Ag3397 相同性解析による交差性が予測される生物種
Full Name CD40 molecule, TNF receptor superfamily member 5
Calculated molecular weight 277 aa, 31 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC012419
Gene Symbol CD40
Gene ID (NCBI) 958
ENSEMBL Gene IDENSG00000101017
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP25942
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD40, also named as TNFRSF5 and Bp50, is a receptor for TNFSF5/CD40LG. It is a member of the TNFR superfamily and is responsible for the efficient activation of cell-mediated and humoral immune responses via activated T cells and B cells respectively.