Anti-Human CD40 (G28.5), PBS Only
CD40 Monoclonal Antibody for FC, Sandwich ELISA, Indirect ELISA
Host / Isotype
Mouse / IgG1, kappa
Reactivity
human
Applications
FC, Sandwich ELISA, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
G28.5
Cat no : 65103-1-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
65103-1-PBS targets CD40 as part of a matched antibody pair:
MP50064-1: 66965-2-PBS capture and 65103-1-PBS detection (validated in Sandwich ELISA)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | N/A 相同性解析による交差性が予測される生物種 |
| Full Name | CD40 molecule, TNF receptor superfamily member 5 |
| Calculated molecular weight | 277 aa, 31 kDa |
| GenBank accession number | BC012419 |
| Gene symbol | CD40 |
| Gene ID (NCBI) | 958 |
| RRID | AB_2881471 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS Only |
| Storage Conditions | Store at -80°C. |
Background Information
CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).
