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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using CD40 Monoclonal antibody (66965-1-Ig)

    WB analysis of Raji using 66965-1-Ig (same clone as 66965-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66965-1-Ig (CD40 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using CD40 Monoclonal antibody (66965-1-Ig)

    WB analysis of Daudi using 66965-1-Ig (same clone as 66965-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66965-1-Ig (CD40 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD40 Monoclonal antibody (66965-1-Ig)

    IHC staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66965-1-Ig (CD40 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD40 Monoclonal antibody (66965-1-Ig)

    IHC staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66965-1-Ig (CD40 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD40 Monoclonal antibody (66965-1-Ig)

    IHC staining of human lymphoma using 66965-1-Ig (same clone as 66965-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human follicular lymphoma tissue slide using 66965-1-Ig (CD40 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD40 Monoclonal antibody (66965-1-Ig)

    IHC staining of human lymphoma using 66965-1-Ig (same clone as 66965-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human follicular lymphoma tissue slide using 66965-1-Ig (CD40 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD40 Monoclonal antibody (66965-1-Ig)

    IF Staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD40 antibody (66965-1-Ig, Clone: 2A8G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD40 Monoclonal antibody (66965-1-Ig)

    IF Staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD40 antibody (66965-1-Ig, Clone: 2A8G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Ramos cells using CD40 Monoclonal antibody (66965-1-Ig)

    IF Staining of Ramos using 66965-1-Ig (same clone as 66965-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Ramos cells using CD40 antibody (66965-1-Ig, Clone: 2A8G5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CD40 Monoclonal antibody (66965-1-Ig)

    IF Staining of Raji using 66965-1-Ig (same clone as 66965-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD40 antibody (66965-1-Ig, Clone: 2A8G5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

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CD40 Monoclonal antibody, PBS Only

CD40 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 66965-1-PBS
Clone No.2A8G5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

p50, CD40L receptor, Bp50, B-cell surface antigen CD40, B cell surface antigen CD40

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66965-1-PBS targets CD40 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD40 fusion protein Ag28040 相同性解析による交差性が予測される生物種
Full Name CD40 molecule, TNF receptor superfamily member 5
Calculated molecular weight 277 aa, 31 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC012419
Gene Symbol CD40
Gene ID (NCBI) 958
ENSEMBL Gene IDENSG00000101017
RRIDAB_2882288
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25942
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cluster of differentiation 40 (CD40) is a costimulatory protein located on antigen presenting cells and is required for their activation. CD40 is a member of the tumor necrosis factor (TNF) receptor (TNFR) family.

What is the molecular weight of CD40?

The molecular weight of CD40 is 43 kDa.

What is the cellular localization of CD40?

CD40 can be secreted by cells or found in the cell membrane.

What is the tissue specificity of CD40?

CD40 is expressed in B cells and primary carcinoma cells but is also found in dendritic cells and macrophages (PMID: 10209159).

What is the function of CD40?

CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.

What is the role of CD40 in disease?

Defects in CD40 lead to hyper-IgM immunodeficiency syndrome type 3 (HIGM3) (PMID: 11675497). This is an autosomal recessive disorder that includes the inability of B cells to undergo isotype switching, a key step in the final differentiation of the humoral immune response, and an inability to mount an antibody-specific immune response.