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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD40L/CD154 Recombinant antibody

CD40L/CD154 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240743F5

Cat no : 83884-3-RR

Synonyms

CD154, Cd40l, Tnfsf5, Cd40lg, CD40 ligand

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD40L/CD154 Recombinant antibody (83884-3-RR)

    WB analysis using 83884-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83884-3-RR (CD40L/CD154 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD40L/CD154 Recombinant antibody (83884-3-RR)

    IHC staining of mouse spleen using 83884-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83884-3-RR (CD40L/CD154 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD40L/CD154 Recombinant antibody (83884-3-RR)

    IHC staining of mouse spleen using 83884-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83884-3-RR (CD40L/CD154 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83884-3-RR

    Affinity and Kinetic Characterization of 83884-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83884-3-RR against Mouse CD40L/CD154 were performed. The affinity constant is 0.406 nM.

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Tested Applications

Positive WB detected inJ774A.1 cells, CTLL-2 cells
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:18000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83884-3-RR targets CD40L/CD154 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD40 ligand
Calculated molecular weight29 kDa
Observed molecular weight32 kDa
GenBank accession numberNM_011616.2
Gene symbol CD154
Gene ID (NCBI) 21947
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33 kDa transmembrane homologue or an 18 kDa soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis.

Protocols

Product Specific Protocols
WB protocol for CD40L/CD154 antibody 83884-3-RRDownload protocol
IHC protocol for CD40L/CD154 antibody 83884-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols