CD40 Recombinant antibody, PBS Only (Capture)

Sandwich ELISA standard curve of MP01294-3, Human CD40 Recombinant Matched Antibody Pair - PBS only. 84406-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1797. 84406-5-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

Saliva of six individual healthy human donors was measured. The CD40 concentration of detected samples was determined to be 829.1 pg/mL with a range of 270.5-2,164.5 pg/mL.

Human milk of five individual healthy human donors was measured. The CD40 concentration of detected samples was determined to be 2,099.7 pg/mL with a range of 1,212.9-3,125.0 pg/mL.

Urine of six individual healthy human donors was measured. The CD40 concentration of detected samples was determined to be 1,6246.6 pg/mL with a range of 5,532.8-21,569.7 pg/mL.

MDA-MB-453 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human CD40, and measured 76.3 pg/mL.

Serum of six individual healthy human donors was measured. The CD40 concentration of detected samples was determined to be 2,121.2 pg/mL with a range of 1,366.2-2,760.0 pg/mL.

Cytometric bead array standard curve of MP01294-2, CD40 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84406-3-PBS. Detection antibody: 84406-4-PBS. Standard: Eg1797. Range: 0.625-80 ng/mL.