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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood platelets using Anti-Human CD42b (AK2) (65163-1-Ig)

    FC experiment of human peripheral blood platelets using 65163-1-Ig

    1X10^6 human peripheral blood platelets were surface co-stained with Anti-Human CD41a labeled with FlexAble CoraLite® Plus488 Antibody Labeling Kit for Mouse IgG1 (KFA021) and 0.2 ug Anti-Human CD42b (65163-1-Ig, Clone:AK2) labeled with FlexAble CoraLite® PlusCL647 Antibody Labeling Kit for Mouse IgG1 (KFA023) or unstained. Cells were not fixed.

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Anti-Human CD42b (AK2)

CD42b Monoclonal Antibody for FC
Cat No. 65163-1-Ig
Clone No.AK2

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Synonyms

AK2, BSS, CD42B, CD42b alpha, GP1B, GP1BA, GPIb alpha, GPIbα

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inhuman peripheral blood platelets

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65163-1-Ig targets CD42b in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Human platelets 相同性解析による交差性が予測される生物種
Full Name glycoprotein Ib (platelet), alpha polypeptide
Calculated molecular weight 626 aa, 69 kDa
GenBank accession numberBC027955
Gene Symbol CD42b
Gene ID (NCBI) 2811
ENSEMBL Gene IDENSG00000185245
RRIDAB_2881480
Conjugate Unconjugated
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
UNIPROT IDP07359
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD42b, also known as platelet glycoprotein Ib alpha chain (GPIb alpha), is a type I transmembrane glycoprotein of 135-145 kDa (PMID: 2656709). It is expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are linked by a disulphide bond to form GPIb (PMID: 7660135). GPIb forms a noncovalent complex with CD42a (GPIX) and CD42d (GPV) and acts as a receptor for von Willebrand factor (vWF) and thrombin (PMID: 7660135; 3759960). The GPIb-V-IX complex mediates vWF-dependent platelet adhesion to blood vessels. Defects in the expression of CD42b result in Bernard-Soulier syndromes and platelet-type von Willebrand disease (PMID: 9371310).

Protocols

Product Specific Protocols
FC protocol for CD42b antibody 65163-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols