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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD42b Monoclonal antibody, PBS Only

CD42b Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G1F5

Cat no : 67857-1-PBS

Synonyms

GP1BA, GP1B, CD42b alpha, BSS, Antigen CD42b-alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood platelets using CD42b Monoclonal antibody (67857-1-Ig)

    WB analysis of human peripheral blood platelets using 67857-1-Ig (same clone as 67857-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67857-1-Ig (CD42b antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67857-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human blood using CD42b Monoclonal antibody (67857-1-Ig)

    WB analysis of human blood using 67857-1-Ig (same clone as 67857-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 67857-1-Ig (CD42b antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67857-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD42b Monoclonal antibody (67857-1-Ig)

    IHC staining of mouse spleen using 67857-1-Ig (same clone as 67857-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67857-1-Ig (CD42b antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67857-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD42b Monoclonal antibody (67857-1-Ig)

    IHC staining of mouse spleen using 67857-1-Ig (same clone as 67857-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67857-1-Ig (CD42b antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67857-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD42b Monoclonal antibody (67857-1-Ig)

    IHC staining of human placenta using 67857-1-Ig (same clone as 67857-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67857-1-Ig (CD42b antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67857-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67857-1-PBS targets CD42b in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD42b fusion protein Ag31181 相同性解析による交差性が予測される生物種
Full Name glycoprotein Ib (platelet), alpha polypeptide
Calculated molecular weight 626 aa, 69 kDa
Observed molecular weight145 kDa
GenBank accession numberBC027955
Gene symbol CD42b
Gene ID (NCBI) 2811
RRIDAB_2918615
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD42b, also named as GP1BA , is a 145-kDa surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. It has a heterodimer form which composed of GP1BA and GP1BB.