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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD43 Monoclonal antibody, PBS Only

CD43 Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A11D6

Cat no : 66224-1-PBS

Synonyms

CD43, Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, sialophorin, SPN

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 66224-1-Ig (same clone as 66224-1-PBS)

    WB analysis of Jurkat using 66224-1-Ig (same clone as 66224-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66224-1-Ig (CD43 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 66224-1-Ig (same clone as 66224-1-PBS)

    WB analysis of K-562 using 66224-1-Ig (same clone as 66224-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66224-1-Ig (CD43 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

    IHC staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD43 antibody included
  • IHC staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

    IHC staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD43 antibody included
  • IHC staining of human appendicitis using 66224-1-Ig (same clone as 66224-1-PBS)

    IHC staining of human appendicitis using 66224-1-Ig (same clone as 66224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD43 antibody included
  • IHC staining of human appendicitis using 66224-1-Ig (same clone as 66224-1-PBS)

    IHC staining of human appendicitis using 66224-1-Ig (same clone as 66224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD43 antibody included
  • IHC staining of human colon using 66224-1-Ig (same clone as 66224-1-PBS)

    IHC staining of human colon using 66224-1-Ig (same clone as 66224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD43 antibody included
  • IHC staining of human colon using 66224-1-Ig (same clone as 66224-1-PBS)

    IHC staining of human colon using 66224-1-Ig (same clone as 66224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD43 antibody included
  • IF Staining of Jurkat using 66224-1-Ig (same clone as 66224-1-PBS)

    IF Staining of Jurkat using 66224-1-Ig (same clone as 66224-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using 66224-1-Ig (CD43 antibody) at dilution of 1:400 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

    IF Staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66224-1-Ig (CD43 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 66224-1-Ig (same clone as 66224-1-PBS)

    FC experiment of Jurkat using 66224-1-Ig (same clone as 66224-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CD43 (66224-1-Ig, Clone:2A11D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy CD43 Ready-To-Use IHC Kit. CD43 primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

66224-1-PBS targets CD43 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD43 fusion protein Ag23595 相同性解析による交差性が予測される生物種
Full Name sialophorin
Calculated molecular weight 400 aa, 43 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC012350
Gene symbol SPN
Gene ID (NCBI) 6693
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.