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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD44 Polyclonal antibody

CD44 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30854-1-AP

Synonyms

CD44, CD44 antigen, CDw44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, HERMES, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, Ly 24, MC56, MDU2, MDU3, MIC4, MUTCH I, Pgp 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD44 Polyclonal antibody (30854-1-AP)

    WB analysis using 30854-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30854-1-AP (Cd44 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD44 Polyclonal antibody (30854-1-AP)

    IF Staining of RAW 264.7 using 30854-1-AP

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD44 antibody (30854-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inJ774A.1 cells, RAW 264.7 cells, NR8383 cells
Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30854-1-AP targets CD44 in WB, IF/ICC, ELISA applications and shows reactivity with Mouse, Rat samples.

Tested Reactivity Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD44 fusion protein Ag33515 相同性解析による交差性が予測される生物種
Full Name CD44 antigen
Calculated molecular weight86kd
Observed molecular weight90 kDa
GenBank accession numberNM_009851
Gene symbol Cd44
Gene ID (NCBI) 12505
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

Protocols

Product Specific Protocols
WB protocol for CD44 antibody 30854-1-APDownload protocol
IF protocol for CD44 antibody 30854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols