Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICC

WB analysis using 60224-1-Ig (same clone as 60224-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
WB analysis using 60224-1-Ig (same clone as 60224-1-PBS)
HeLa (CD44+), A549 (CD44+), MDA-MB-231 (CD44+), MCF-7, Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
WB analysis of HeLa cells using 60224-1-Ig (same clone as 60224-1-PBS)
WB result of CD44 antibody (60224-1-Ig, 1:4000) with si-Control and si-CD44 transfected HeLa cells. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
WB analysis of human spleen using 60224-1-Ig (same clone as 60224-1-PBS)
human spleen tissue were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 60224-1-Ig (same clone as 60224-1-PBS)
IP result of anti-CD44 (IP:60224-1-Ig, 4ug; Detection:60224-1-Ig 1:3000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human oesophagus cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human oesophagus cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IF Staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 60224-1-Ig (CD44 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IF Staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 60224-1-Ig (CD44 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IF Staining of A431 using 60224-1-Ig (same clone as 60224-1-PBS)
Immunofluorescent analysis of un-fixed A431 cells using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:1800 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.
IF Staining of A431 using 60224-1-Ig (same clone as 60224-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

CD44 Monoclonal antibody, PBS Only

CD44 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 60224-1-PBS

Clone No.6F4H2

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

ECMR-III, ECMR III, CSPG8, CDW44, CD44 antigen

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60224-1-PBS targets CD44 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity	human, pig
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CD44 fusion protein Ag7633 相同性解析による交差性が予測される生物種
Full Name	CD44 molecule (Indian blood group)
Calculated molecular weight	742 aa, 82 kDa
Observed molecular weight	80-95 kDa
GenBank accession number	BC004372
Gene Symbol	CD44
Gene ID (NCBI)	960
ENSEMBL Gene ID	ENSG00000026508
RRID	AB_11042767
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P16070
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 60224-1-Ig (same clone as 60224-1-PBS)

WB analysis using 60224-1-Ig (same clone as 60224-1-PBS)

WB analysis of HeLa cells using 60224-1-Ig (same clone as 60224-1-PBS)

WB analysis of human spleen using 60224-1-Ig (same clone as 60224-1-PBS)

IP experiment of HeLa using 60224-1-Ig (same clone as 60224-1-PBS)

IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

IHC staining of human oesophagus cancer using 60224-1-Ig (same clone as 60224-1-PBS)

IHC staining of human oesophagus cancer using 60224-1-Ig (same clone as 60224-1-PBS)

IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

IHC staining of human tonsillitis using 60224-1-Ig (same clone as 60224-1-PBS)

IHC staining of human tonsillitis using 60224-1-Ig (same clone as 60224-1-PBS)

IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

IHC staining of human breast cancer using 60224-1-Ig (same clone as 60224-1-PBS)

IHC staining of human breast cancer using 60224-1-Ig (same clone as 60224-1-PBS)

IF Staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

IF Staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

IF Staining of A431 using 60224-1-Ig (same clone as 60224-1-PBS)

IF Staining of A431 using 60224-1-Ig (same clone as 60224-1-PBS)

CD44 Monoclonal antibody, PBS Only

CD44 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information