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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using Anti-Human CD45 Rabbit Recombinant Antibody (98117-1-RR)

    IF Staining of Jurkat using 98117-1-RR (same clone as 98117-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45 antibody (98117-1-RR, Clone: 241670E10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 98117-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human CD45 Rabbit Recombinant Antibody (98117-1-RR)

    FC experiment of human peripheral blood leukocytes using 98117-1-RR (same clone as 98117-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD45 Rabbit Recombinant Antibody (98117-1-RR, Clone: 241670E10) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98117-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98117-1-RR

    Affinity and Kinetic Characterization of 98117-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98117-1-RR against Human CD45 were performed. The affinity constant is 2.58 nM.

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Anti-Human CD45 Rabbit Recombinant Antibody, PBS Only

CD45 Uni-rAbTM Recombinant Antibody for IF/ICC, FC
Cat No. 98117-1-PBS
Clone No.241670E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC

Synonyms

LCA, L CA, GP180, EC:3.1.3.48, B220

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98117-1-PBS targets CD45 in IF/ICC, FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
Gene Symbol CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).