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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD45 Polyclonal antibody

CD45 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29914-1-AP

Synonyms

LCA, L CA, GP180, EC:3.1.3.48, CD45R

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD45 Polyclonal antibody (29914-1-AP)

    WB analysis using 29914-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29914-1-AP (CD45 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD45 Polyclonal antibody (29914-1-AP)

    IHC staining of human tonsillitis using 29914-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 29914-1-AP (CD45 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD45 Polyclonal antibody (29914-1-AP)

    IHC staining of human tonsillitis using 29914-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 29914-1-AP (CD45 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using CD45 Polyclonal antibody (29914-1-AP)

    IHC staining of human colon using 29914-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29914-1-AP (CD45 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using CD45 Polyclonal antibody (29914-1-AP)

    IHC staining of human colon using 29914-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29914-1-AP (CD45 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD45 Polyclonal antibody (29914-1-AP)

    IHC staining of human liver cancer using 29914-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 29914-1-AP (CD45 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD45 Polyclonal antibody (29914-1-AP)

    IHC staining of human liver cancer using 29914-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 29914-1-AP (CD45 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, Raji cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue, human liver cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29914-1-AP targets CD45 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD45 fusion protein Ag31286 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
Calculated molecular weight 1304 aa, 147 kDa
Observed molecular weight180-250 kDa
GenBank accession numberBC014239
Gene symbol CD45
Gene ID (NCBI) 5788
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single-chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

Protocols

Product Specific Protocols
WB protocol for CD45 antibody 29914-1-APDownload protocol
IHC protocol for CD45 antibody 29914-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols