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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD45 Polyclonal antibody

CD45 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31243-1-AP

Synonyms

Ptprc, Leukocyte common antigen, LCA, B220

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD45 Polyclonal antibody (31243-1-AP)

    WB analysis using 31243-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31243-1-AP (CD45/PTPRC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD45 Polyclonal antibody (31243-1-AP)

    IF Staining of RAW 264.7 using 31243-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CD45 antibody (31243-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inEL-4 cells, J774A.1 cells, RAW 264.7 cells, mouse spleen tissue, mouse thymus tissue
Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31243-1-AP targets CD45 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
Calculated molecular weight145 kDa
Observed molecular weight180 kDa
GenBank accession numberNM_001111316.2
Gene symbol CD45
Gene ID (NCBI) 19264
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Protocols

Product Specific Protocols
WB protocol for CD45 antibody 31243-1-APDownload protocol
IF protocol for CD45 antibody 31243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols