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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD45 (HI30)

CD45 Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

IF/ICC, FC

Conjugate

Unconjugated

CloneNo.

HI30

Cat no : 65109-1-Ig

Synonyms

L CA, HI30, GP180, EC:3.1.3.48, CD45R

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using Anti-Human CD45 (HI30) (65109-1-Ig)

    IF Staining of Jurkat using 65109-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45 antibody (65109-1-Ig, Clone: HI30 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD45 (HI30) (65109-1-Ig)

    FC experiment of human PBMCs using 65109-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD45 (65109-1-Ig, Clone:HI30) or Mouse IgG1 Isotype Control and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:500. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive IF/ICC detected inJurkat cells
Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65109-1-Ig targets CD45 in IF/ICC, FC applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human peripheral blood leucocytes 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
GenBank accession numberBC014239
Gene symbol CD45
Gene ID (NCBI) 5788
RRIDAB_2918407
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Protocols

Product Specific Protocols
IF protocol for CD45 antibody 65109-1-IgDownload protocol
FC protocol for CD45 antibody 65109-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

Front Cell Dev Biol

Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction.

Authors - Xun Xi
View Article
humanIF

Cell Discov

Integration of 3D bioprinting and multi-algorithm machine learning identified glioma susceptibilities and microenvironment characteristics

Authors - Min Tang
View Article