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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD45 Recombinant antibody (80297-1-RR)

    WB analysis using 80297-1-RR (same clone as 80297-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80297-1-RR (CD45 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using CD45 Recombinant antibody (80297-1-RR)

    IHC staining of mouse colon using 80297-1-RR (same clone as 80297-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using CD45 Recombinant antibody (80297-1-RR)

    IHC staining of mouse colon using 80297-1-RR (same clone as 80297-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD45 Recombinant antibody (80297-1-RR)

    IHC staining of human appendicitis using 80297-1-RR (same clone as 80297-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD45 Recombinant antibody (80297-1-RR)

    IHC staining of human appendicitis using 80297-1-RR (same clone as 80297-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD45 Recombinant antibody (80297-1-RR)

    IHC staining of human tonsillitis using 80297-1-RR (same clone as 80297-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD45 Recombinant antibody (80297-1-RR)

    IF Staining of mouse spleen using 80297-1-RR (same clone as 80297-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CD45 antibody (80297-1-RR, Clone: 6O19 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD45 Recombinant antibody (80297-1-RR)

    IF Staining of mouse spleen using 80297-1-RR (same clone as 80297-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CD45 antibody (80297-1-RR, Clone: 6O19 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using CD45 Recombinant antibody (80297-1-RR)

    IF Staining of mouse colon using 80297-1-RR (same clone as 80297-1-PBS)

    Immunofluorescence of mouse colon: Frozen OCT-embedded mouse colon sections were stained with anti-E-cadherin antibody (20874-1-AP) labeled with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2, green) in the frist step,anti-CD45 antibody (80297-1-RR) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, orange) in the second step,anti-Collagen Type III antibody (22734-1-AP) labeled with FlexAble CoraLite647 Kit (KFA003, magenta) in the third step, and DAPI (blue). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD45 Recombinant antibody (80297-1-RR)

    IF Staining of Jurkat using 80297-1-RR (same clone as 80297-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45 antibody (80297-1-RR, Clone: 6O19 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80297-1-RR

    Affinity and Kinetic Characterization of 80297-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80297-1-RR against Human CD45 were performed. The affinity constant is 1.61 nM.

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CD45 Recombinant antibody, PBS Only

CD45 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA
Cat No. 80297-1-PBS
Clone No.6O19

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Synonyms

LCA, L CA, GP180, EC:3.1.3.48, B220

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80297-1-PBS targets CD45 in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CD45 fusion protein Ag13712 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
Calculated molecular weight 1304 aa, 147 kDa
Observed molecular weight220-240 kDa
GenBank accession numberBC014239
Gene Symbol CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_2918884
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08575
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.