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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD45 Recombinant antibody (84405-6-RR)

    WB analysis of Jurkat using 84405-6-RR (same clone as 84405-6-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 84405-6-RR (CD45 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84405-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD45 Recombinant antibody (84405-6-RR)

    IHC staining of human tonsillitis using 84405-6-RR (same clone as 84405-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84405-6-RR (CD45 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84405-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD45 Recombinant antibody (84405-6-RR)

    IHC staining of human tonsillitis using 84405-6-RR (same clone as 84405-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84405-6-RR (CD45 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84405-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD45 Recombinant antibody (84405-6-RR)

    IF Staining of Jurkat using 84405-6-RR (same clone as 84405-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45(220-577) antibody (84405-6-RR, Clone: 241670B6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84405-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CD45 Recombinant antibody (84405-6-RR)

    IF Staining of Raji using 84405-6-RR (same clone as 84405-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD45(220-577) antibody (84405-6-RR, Clone: 241670B6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84405-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84405-6-RR

    Affinity and Kinetic Characterization of 84405-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84405-6-RR against Human CD45 were performed. The affinity constant is 3.91 nM.

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CD45 Recombinant antibody, PBS Only

CD45 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84405-6-PBS
Clone No.241670B6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

LCA, L CA, GP180, EC:3.1.3.48, B220

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84405-6-PBS targets CD45 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
Gene Symbol CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).