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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse CD45 Recombinant antibody, PBS Only (Capture)

CD45 Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240920B4

Cat no : 83396-14-PBS

Synonyms

Ptprc, Leukocyte common antigen, LCA, B220, 240920B4

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00412-4

    Sandwich ELISA standard curve of MP00412-4

    Sandwich ELISA standard curve of MP00412-4, Mouse CD45 Recombinant Matched Antibody Pair - PBS only. 83396-14-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0845. 83396-7-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL.

  • Sample test of MP00412-4

    Sample test of MP00412-4

    The mean mouse CD45 concentration was determined to be 39.3 ng/mL in RAW 264.7 cell extract based on a 4.8 mg/mL extract load.

  • Sample test of MP00412-4

    Sample test of MP00412-4

    RAW 264.7 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for mouse CD45, and measured 154.9 pg/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83396-14-PBS targets CD45 as part of a matched antibody pair:

MP00412-4: 83396-14-PBS capture and 83396-7-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
Calculated molecular weight145 kDa
GenBank accession numberNM_001111316.2
Gene symbol CD45
Gene ID (NCBI) 19264
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.