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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD45RA (HI100), PBS Only

CD45RA Monoclonal Antibody for Indirect ELISA

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human

Applications

Indirect ELISA

Conjugate

Unconjugated

CloneNo.

HI100

Cat no : 65108-1-PBS

Synonyms

CD45, B220, HI100, GP180, EC:3.1.3.48

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD45RA (HI100) (65108-1-Ig)

    FC experiment of human PBMCs using 65108-1-Ig (same clone as 65108-1-PBS)

    1X10^6 human PBMCs were surface co-stained with CL647 Anti-Human CD45RO and 0.2 ug Anti-Human CD45RA (65108-1-Ig, Clone:HI100) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65108-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD45RA (HI100) (65108-1-Ig)

    FC experiment of human PBMCs using 65108-1-Ig (same clone as 65108-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD45RA (65108-1-Ig, Clone:HI100) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red) or unstained. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65108-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65108-1-PBS targets CD45RA in Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
GenBank accession numberBC014239
Gene symbol CD45
Gene ID (NCBI) 5788
RRIDAB_2881472
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).