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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD45RA (F8-11-13)

CD45RA Monoclonal Antibody for IHC, IF-P, FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

IHC, IF-P, FC

Conjugate

Unconjugated

CloneNo.

F8-11-13

Cat no : 65226-1-Ig

Synonyms

CD45, B220, L CA, GP180, F8-11-13

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Anti-Human CD45RA (F8-11-13) (65226-1-Ig)

    IHC staining of human tonsillitis using 65226-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 65226-1-Ig (CD45RA antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using Anti-Human CD45RA (F8-11-13) (65226-1-Ig)

    IF Staining of human tonsillitis using 65226-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD45RA antibody (65226-1-Ig, Clone: F8-11-13 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD45RA (F8-11-13) (65226-1-Ig)

    FC experiment of human PBMCs using 65226-1-Ig

    1X10^6 human PBMCs were surface co-stained with PE Anti-human CD3, FITC Anti-human CD45RO, and 0.2 ug Anti-Human CD45RA (65226-1-Ig, Clone: F8-11-13) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG1 (KFA023) or Mouse IgG1 Isotype Control. Cells were not fixed. CD3+ lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD45RA (F8-11-13) (65226-1-Ig)

    FC experiment of human PBMCs using 65226-1-Ig

    1X10^6 human PBMCs were surface co-stained with PE Anti-human CD3 and 0.2 ug Anti-Human CD45RA (65226-1-Ig, Clone: F8-11-13) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG1 (KFA023) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. CD3+ lymphocytes were gated.

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Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:1000-1:4000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65226-1-Ig targets CD45RA in IHC, IF-P, FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human T lymphocytes 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
Calculated molecular weight 1304 aa, 147 kDa
GenBank accession numberBC014239
Gene symbol CD45
Gene ID (NCBI) 5788
RRIDAB_3084907
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

Protocols

Product Specific Protocols
IHC protocol for CD45RA antibody 65226-1-IgDownload protocol
IF protocol for CD45RA antibody 65226-1-IgDownload protocol
FC protocol for CD45RA antibody 65226-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols