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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD45RA (F8-11-13)

CD45RA Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

F8-11-13

Cat no : 65226-1-Ig

Synonyms

CD45, B220, GP180, F8-11-13, EC:3.1.3.48

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD45RA (F8-11-13) (65226-1-Ig)

    FC experiment of human PBMCs using 65226-1-Ig

    1X10^6 human PBMCs were surface co-stained with PE Anti-human CD3, FITC Anti-human CD45RO, and 0.2 ug Anti-Human CD45RA (65226-1-Ig, Clone: F8-11-13) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG1 (KFA023) or Mouse IgG1 Isotype Control. Cells were not fixed. CD3+ lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD45RA (F8-11-13) (65226-1-Ig)

    FC experiment of human PBMCs using 65226-1-Ig

    1X10^6 human PBMCs were surface co-stained with PE Anti-human CD3 and 0.2 ug Anti-Human CD45RA (65226-1-Ig, Clone: F8-11-13) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG1 (KFA023) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. CD3+ lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65226-1-Ig targets CD45RA in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human T lymphocytes 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
Calculated molecular weight 1304 aa, 147 kDa
GenBank accession numberBC014239
Gene symbol CD45
Gene ID (NCBI) 5788
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

Protocols

Product Specific Protocols
FC protocol for CD45RA antibody 65226-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols