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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD46 Polyclonal antibody (28936-1-AP)

    WB analysis using 28936-1-AP (same clone as 28936-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 28936-1-AP (CD46 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD46 Polyclonal antibody (28936-1-AP)

    WB analysis using 28936-1-AP (same clone as 28936-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 28936-1-AP (CD46 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CD46 Polyclonal antibody (28936-1-AP)

    WB analysis of Jurkat using 28936-1-AP (same clone as 28936-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 28936-1-AP (CD46 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD46 Polyclonal antibody (28936-1-AP)

    IHC staining of human colon cancer using 28936-1-AP (same clone as 28936-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28936-1-AP (CD46 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD46 Polyclonal antibody (28936-1-AP)

    IHC staining of human colon cancer using 28936-1-AP (same clone as 28936-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28936-1-AP (CD46 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD46 Polyclonal antibody (28936-1-AP)

    IF Staining of HeLa using 28936-1-AP (same clone as 28936-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CD46 antibody (28936-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

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CD46 Polyclonal antibody, PBS Only

CD46 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28936-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

TRA2.10, TLX, MIC10, Membrane cofactor protein, MCP

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28936-1-PBS targets CD46 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD46 fusion protein Ag30376 相同性解析による交差性が予測される生物種
Full Name CD46 molecule, complement regulatory protein
Calculated molecular weight 384 aa, 43 kDa
Observed molecular weight50-70 kDa
GenBank accession numberBC030594
Gene Symbol CD46
Gene ID (NCBI) 4179
RRIDAB_2881233
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15529
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CD46, also known as Membrane cofactor protein (MCP) or MIC10, is a ubiquitously expressed type type-I transmembrane glycoprotein that acts as a cofactor for complement factor I to mediate inactivation of C3b and C4b deposited on host cells. CD46 is a receptor for a large number of pathogens, including measles virus and adenovirus (PMID: 20941397). CD46 plays a role in linking innate and adaptive immune responses. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations of the CD46 gene have been associated with some diseases including hemolytic uremic syndrome (PMID: 26054645). CD46 is expressed on most cells as four isoforms that are derived via alternative splicing (PMID: 15324743).