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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD47 Polyclonal antibody, PBS Only

CD47 Polyclonal Antibody for IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20305-1-PBS

Synonyms

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CD47 Polyclonal antibody (20305-1-AP)

    IHC staining of human prostate cancer using 20305-1-AP (same clone as 20305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20305-1-AP (CD47 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20305-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CD47 Polyclonal antibody (20305-1-AP)

    IHC staining of human prostate cancer using 20305-1-AP (same clone as 20305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20305-1-AP (CD47 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20305-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CD47 Polyclonal antibody (20305-1-AP)

    IHC staining of human ovary tumor using 20305-1-AP (same clone as 20305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20305-1-AP (CD47 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20305-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CD47 Polyclonal antibody (20305-1-AP)

    IHC staining of human ovary tumor using 20305-1-AP (same clone as 20305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20305-1-AP (CD47 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20305-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20305-1-PBS targets CD47 in IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD47 fusion protein Ag14132 相同性解析による交差性が予測される生物種
Full Name CD47 molecule
Calculated molecular weight 323 aa, 35 kDa
GenBank accession numberBC010016
Gene symbol CD47
Gene ID (NCBI) 961
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts as a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.